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Application Note ReView: AS240: Automated Extraction of Free Base and Protonated Nicotine From E-Liquids


9th November 2021

e-Liquids, Extraction, Nicotine, Sample Prep,

e-liquids, nicotine, vape, e-cigarette


With the announcement that licensed e-cigarettes may soon be available via the NHS in order to help smokers to quit smoking, this edition of App Note ReView takes a look at AS240 – Automated Extraction of Free Base and Protonated Nicotine From E-Liquid Samples.

The determination of free-base and protonated nicotine in e-liquids is a requirement of the Tobacco Products Directive and is usually carried out via manual solvent extraction and gas chromatography analysis.

This Application Note describes an automated extraction procedure using the GERSTEL MPSrobotic platform, as well as automated preparation of calibration standards, on e-liquid samples.

The automated calibration preparation shows better linearity – 0.9997 vs. 0.9977 – than the manual procedure and the sample extraction compares favourably to the manual procedure.

An additional benefit is the significant reduction in analyst time, with the only manual part of the process being the initial addition of the e-liquid samples to the extraction vials.


CLICK HERE to download the full AS240: Automated Extraction of Free Base and Protonated Nicotine From E-Liquids Application Note



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