AS240: Automated Extraction of Free Base and Protonated Nicotine From E-Liquids
The determination of free-base and protonated nicotine in e-liquids is a requirement of the Tobacco Products Directive and is usually carried out via manual solvent extraction and gas chromatography analysis.
This Application Note describes an automated extraction procedure, using the GERSTEL MPSrobotic platform, as well as automated preparation of calibration standards.
The automated calibration preparation shows better linearity – 0.9997 vs. 0.9977 – than the manual procedure and the sample extraction compares favourably to the manual procedure.
An additional benefit is the significant reduction in analyst time, with the only manual part of the process being the initial addition of the e-liquid samples to the extraction vials.
Anatune Application Notes:
Anatune (and our partners) regularly produce Application Notes. These cover a wide and diverse range of methods, techniques, applications, and related chromatography technology. Our Application Notes provide detailed technical information, unique applications insight and method development & validation, as well as systems configuration innovations to ensure readers can further their knowledge in an engaging way. For many years, Anatune’s Application Notes have helped and supported analytical chemists in the fields of Gas Chromatography – GC, Liquid Chromatography – LC, and Mass Spectrometry – MS achieve to develop analytical solutions. Visit our Applications Note page to search for the insight you need in the following categories: Matrix, Technique, Analyte, Instruments, Product, Industry. Or alternatively, CONTACT US for more information or to put forward an Application Note subject and our Applications Team will be happy to support you in any way they can.
Before you download...
Please enter your email address before downloading this document.