GME8: Automating Metabolic Stability Assays and Analyses Using a Robotic Autosampler and LC-MS/MS Platform
Automating Metabolic Stability Assays and Analyses Using a Robotic Autosampler and LC-MS/MS Platform:
The liver is the principal organ involved in mammalian metabolism of xenobiotics including drug compounds. Microsomes are isolated through differential centrifugation of liver tissue homogenate and are principally derived from the membranes of the endoplasmic reticulum. These microsomes provide an enriched source of membrane bound drug metabolising enzymes including the cytochrome P450 (CYP) superfamily and uridine glucuronosyl transferase (UGT) enzymes. To study and assess the metabolism of chemical drug compounds, DMPK laboratories can perform in vitro studies using liver microsomes.
As a result of this study, we were able to show that an in vitro metabolic stability assay and subsequent sample preparation method can be successfully automated using the GERSTEL MPS robotic sampler for a variety of model drug compounds in microsomes. Using this method, analytes can be rapidly and reproducibly isolated from microsome samples using an automated procedure coupled to LC-MS/MS analysis using the Agilent Ultivo Triple Quadrupole Mass Spectrometer.
Anatune Application Notes:
Anatune (and our partners) regularly produce Application Notes. These cover a wide and diverse range of methods, techniques, applications, and related chromatography technology. Our Application Notes provide detailed technical information, unique applications insight and method development & validation, as well as systems configuration innovations to ensure readers can further their knowledge in an engaging way. For many years, Anatune’s Application Notes have helped and supported analytical chemists in the fields of Gas Chromatography – GC, Liquid Chromatography – LC, and Mass Spectrometry – MS achieve to develop analytical solutions. Visit our Applications Note page to search for the insight you need in the following categories: Matrix, Technique, Analyte, Instruments, Product, Industry. Or alternatively, CONTACT US for more information or to put forward an Application Note subject and our Applications Team will be happy to support you in any way they can.
Before you download...
Please enter your email address before downloading this document.