AS222: Automated Extraction and Derivatisation for the Detection of Steroids in Blood Using Mitra® Microsampling Devices

Martin Perkins

26th June 2019

Blood microsampling for automated steroid analysis offers a very appealing alternative to current anti-doping procedures as it is significantly less invasive and does not require specialists for sample collection. Samples are easy to transport, are stable over extended periods of time and cheaper to both use and store.

Recently, a new blood microsampling technology has emerged; the Mitra® microsampling device, driven by Volumetric Absorptive Microsampling (VAMS™) technology.

This application note describes the optimisation of an automated steroid analysis workflow for the extraction, derivatisation and analysis of steroids from Mitra® devices for anti-doping purposes. The optimised automated method has been compared to the existing manual method to evaluate recoveries and assess method performance. Recoveries ranged between 84% -146% for the manual method and 77%-128% for automation. Good agreement was observed between the manual and automated approach.

**********************

Anatune Application Notes:

Anatune (and our partners) regularly produce Application Notes. These cover a wide and diverse range of methods, techniques, applications, and related chromatography technology. Our Application Notes provide detailed technical information, unique applications insight and method development & validation, as well as systems configuration innovations to ensure readers can further their knowledge in an engaging way. For many years, Anatune’s Application Notes have helped and supported analytical chemists in the fields of Gas Chromatography – GC, Liquid Chromatography – LC, and Mass Spectrometry – MS achieve to develop analytical solutions. Visit our Applications Note page to search for the insight you need in the following categories: Matrix, Technique, Analyte, Instruments, Product, Industry. Or alternatively, CONTACT US for more information or to put forward an Application Note subject and our Applications Team will be happy to support you in any way they can.