AS232: Dispersive Liquid-Liquid MicroExtraction (DiLLME) for Whisky Flavour Analysis

Martin Perkins

22nd January 2020

Measurement of the volatile profiles of alcoholic beverages, such as whisky flavour analysis, are important both for understanding flavour characteristics and investigating counterfeit products. Compounds responsible may be present at extremely low levels, resulting in the need for sensitive and robust methods.

There are a number of techniques that can be employed for the analysis of flavours from liquid samples, many of which can be fully automated. Automated liquid extraction can be used as an alternative to more established flavour profiling techniques, generally based on headspace extraction. This application note describes the use of dispersive liquid-liquid microextraction (DiLLME) for flavour analysis in Whisky. DiLLME has advantages over other approaches due to its simplicity and speed. Furthermore, as unlike headspace approaches the sample does not need to be heated, it has less potential for formation of compounds and potentially less volatile compounds can be analysed.

A number of commercially available Whisky samples were analysed and data processed using Agilent MassHunter and Mass Profiler Professional software. The resultant principal component analysis (PCA) was able to separate the Whisky samples and the compounds responsible for some of the distinct flavours could be determined from the data.

This work demonstrates that DiLLME is a suitable technique to extract a range of compounds relevant to flavour in Whiskies. Compounds observed included esters, Whisky lactones, phenolic compounds and aldehydes, as well as less volatile compounds associated with the ageing process, such as Homovanillic acid and Syringylacetone.



Element Application Notes:

Element (and our partners) regularly produce Application Notes. These cover a wide and diverse range of methods, techniques, applications, and related chromatography technology. Our Application Notes provide detailed technical information, unique applications insight and method development & validation, as well as systems configuration innovations to ensure readers can further their knowledge in an engaging way. For many years, Element’s Application Notes have helped and supported analytical chemists in the fields of Gas Chromatography – GC, Liquid Chromatography – LC, and Mass Spectrometry – MS achieve to develop analytical solutions. Visit our Applications Note page to search for the insight you need in the following categories: Matrix, Technique, Analyte, Instruments, Product, Industry. Or alternatively, CONTACT US for more information or to put forward an Application Note subject and our Applications Team will be happy to support you in any way they can.



Before you download...

Please enter your email address before downloading this document.