AS104: Automating the Analysis of Androstenedione and Testosterone in Serum Using a GERSTEL MultiPurpose Sampler Coupled to Liquid Chromatography Tandem Mass Spectrometry Detection

Martin Perkins

13th September 2019


The measurement of testosterone and androstenedione in female patients is usually performed to assess androgen excess which can cause several hormonal disorders. There are many causes of androgen excess mostly related to endocrine and iatrogenic causes.

Historically, mostly immunoassays have been used to measure these analytes. However, immunoassays, particularly for testosterone, are prone to interference from related steroids and steroid like compounds.

This study describes the development and validation of an automated SPE-LC-MS/MS method which offered higher sensitivity and selectivity compared to the immunoassays.

The fully automated sample preparation features internal standard and reagent addition, protein precipitation and centrifugation, coupled directly to LC-MS/MS. The system is capable of handling 98 samples in 13 hours and 15 minutes.

 

**********************

Element Application Notes:

Element (and our partners) regularly produce Application Notes. These cover a wide and diverse range of methods, techniques, applications, and related chromatography technology. Our Application Notes provide detailed technical information, unique applications insight and method development & validation, as well as systems configuration innovations to ensure readers can further their knowledge in an engaging way. For many years, Element’s Application Notes have helped and supported analytical chemists in the fields of Gas Chromatography – GC, Liquid Chromatography – LC, and Mass Spectrometry – MS achieve to develop analytical solutions. Visit our Applications Note page to search for the insight you need in the following categories: Matrix, Technique, Analyte, Instruments, Product, Industry. Or alternatively, CONTACT US for more information or to put forward an Application Note subject and our Applications Team will be happy to support you in any way they can.

 

Downloads

Before you download...

Please enter your email address before downloading this document.