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Vitamin D LC/MS/MS Analyser

A fully automated analyser was developed featuring protein crash step, solid phase extraction (SPE) and LC-MS/MS analysis

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Application Notes

A fully automated solution was developed featuring protein crash step, solid phase extraction (SPE) and LC-MS/MS analysis. The method fully separates 25-OH-D3/D2 from the inactive form Epi-25-OH-D3/D2. The comprehensive prep ahead capability in Maestro software allows the system to fully process and analyse 96 samples in just under seventeen hours. This offers significant advantages when compared to a standard manual approach requiring sample preparation with subsequent LC-MS analysis.